Hayashi, Kotaro

Hayashi, Kotaro


Kataoka/Kinoh Lab

Job title

Research Scientist


Ph. D. in Engineering

Other affiliation

Visiting Researcher, Department of Material Engineering, Graduate School of Engineering, The University of Tokyo


Research contents (Research key words)

Drug delivery system, Nucleic acid medicine, Supramolecular Chemistry, Functional polymer chemisitry


  • 2010.03

    B. Sci. Department of Chemistry, School of Science, The University of Tokyo.

  • 2012.03

    M. Sci. Department of Chemistry, Graduate School of Science, The University of Tokyo

  • 2016.03

    Ph. D. (Eng.) Department of Bioengineering, Graduate School of Engineering, The University of Tokyo
    Doctoral Dissertation: Preparation and characterization of unit polyion complexe with siRNA utilizing PEG-block-poly(amino acid)s

  • 2014.04-2016.03

    JSPS Research Fellow (DC2), The University of Tokyo

  • 2016.04-Present

    Research Scientist, Innovation Center of NanoMedicine


  1. S. Watanabe, K. Hayashi, K. Toh, H. J. Kim, X. Liu, H. Chaya, S. Fukushima, K. Katsushima, Y. Kondo, S. Uchida, S. Ogura, T. Nomoto, H. Takemoto, H. Cabral, H. Kinoh, H. Tanaka, M. R. Kano, Y. Matsumoto, H. Fukuhara, S. Uchida, M. Nangaku, K. Osada, N. Nishiyama, K. Miyata, K. Kataoka, In vivo rendezvous of small nucleic acid drugs with charge-matched block catiomers to target cancer. Nat. Commun. 10 1894 (2019)
  2. T. Takai, T. Tsujino, Y. Yoshikawa, T. Inamoto, N. Sugito, Y. Kuranaga, T. Soga, K. Hayashi, K. Miyata, K. Kataoka, H. Azuma, Y. Akao, Synthetic miR-143 exhibited an anti-cancer effect via downregulation of K-RAS networks of renal cell cancer cells in vitro and in vivo. Mol. Ther. 27 (5) 1-11 (2019)
  3. Y. Yoshikawa, K. Taniguchi, T. Tsujino, K. Heishima, T. Inamoto, T. Takai, K. Minami, H. Azuma, K. Miyata, K. Hayashi, K. Kataoka, Y. Akao, Anti-cancer effects of a chemically modified miR-143 on bladder cancer by either systemic or intravesical treatment. Mol. Ther. Methods Clin. Dev. 13 290-302 (2019)
  4. B. -S. Kim, S. Chuanoi, T. Suma, Y. Anraku, K. Hayashi, M. Naito, H. -J. Kim, I. C. Kwon, K. Miyata, A. Kishimura, K. Kataoka, Self-assembly of siRNA/PEG-b-catiomer at integer molar ratio into 100 nm-sized vesicular polyion complexes (siRNAsomes) for RNAi and codelivery of cargo macromolecules. J. Am. Chem. Soc. 141 (8) 3699-3709 (2019)
  5. M. Naito, Y. Otsu, R. Kamegawa, K. Hayashi, S. Uchida, H. -J. Kim, K. Miyata, Tunable non-enzymatic degradability of N-substituted polyaspartamide main chain by amine protonation and alkyl spacer length in side chains for enhanced messenger RNA transfection efficiency. Sci. Technol. Adv. Mater. 20 (1) 105-115 (2019)
  6. Y. Yi, H. J. Kim, M. Zheng, P. Mi, M. Naito, B. S. Kim, H. S. Min, K. Hayashi, F. Perche, K. Toh, X. Liu, Y. Mochida, H. Kinoh, H. Cabral, K. Miyata, K. Kataoka, Glucose-linked sub-50-nm unimer polyion complex-assembled gold nanoparticles for targeted siRNA delivery to glucose transporter 1-overexpressing breast cancer stem-like cells. J. Control. Release 295 268-277 (2019)
  7. H. -S. Min, H. -J. Kim, J. -Y. Ahn, M. Naito, K. Hayashi, K. Toh, B. -S. Kim, Y. Matsumura, I.-C. Kwon, K. Miyata, K. Kataoka, Tuned density of anti-tissue factor antibody fragment onto siRNA-loaded polyion complex micelle for optimizing targetability into pancreatic cancer cells. Biomacromolecules 19 (6) 2320-2329 (2018)
  8. Y. Yi, H. -J. Kim, P. Mi, M. Zheng, H. Takemoto, K. Toh, B. -S. Kim, K. Hayashi, M. Naito, Y. Matsumoto, K. Miyata, K. Kataoka, Targeted systemic delivery of siRNA to cervical cancer model using cyclic RGD-installed unimer polyion complex-assembled gold nanoparticles. J. Control. Release 244 PartB 247-256 (2016)
  9.  K. Hayashi, H. Chaya, S. Fukushima, S. Watanabe, H. Takemoto, K. Osada, N. Nishiyama, K. Miyata, K. Kataoka, Influence of RNA strand rigidity on polyion complex formation with block catiomers. Macromol. Rapid Commun. 37 (6) 486-493 (2016) 
  10. H. -J. Kim, H. Takemoto, Y. Yi, M. Zheng, Y. Maeda, H. Chaya, K. Hayashi, P. Mi, F. Pittella, R. J. Christie, K. Toh, Y. Matsumoto, N. Nishiyama, K. Miyata, K. Kataoka, Precise engineering of siRNA delivery vehicles to tumors using polyion complexes and gold nanoparticles. ACS Nano 8 (9) 8979-8991 (2014)

Research Funding

  • 2014-2015 Grant-in-Aid for JSPS Fellow
  • 2016-2017 Grant-in-Aid for Research Activity start-up
  • 2018-2020 Grant-in-Aid for Young Scientists

Affiliation Society

  • American Chemical Society
  • The Chemical Society of Japan
  • The Society of Polymer Science, Japan
  • The Japan Society of Drug Delivery System