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Ueno, Shingo

Ueno, Shingo

Section

Ichiki Lab.

Job title

Deputy Principal Research Scientist

Degree

Ph.D.

Other affiliation

Visiting Researcher, Department of Materials Engineering, The University of Tokyo

URL

Ichiki Lab, Researchmap

Research contents (Research key words)

High functional properties of biomolecules such as enzymes, antibodies, peptides, and nucleic acids are widely used in medical and other various fields. Development or improvement of these biomolecules assist the solution of global issues relating health and medical, food, energy and so on. Functionality of biomolecules have been improved with the evolution of the organisms on the earth. I am developing the technologies that enable to improve or create the functional biomolecules by applying the natural selection theory to biotechnology. Currently, I am focusing on the development of the system for artificial selection of biomolecules based on microarray technologies.

Keywords

Evolutionary Molecular Engineering, In Vitro Selection, Microarray

Biography

Education

  • Sep. 2007

    Ph.D. in Bioenvironmental Science from Saitama Univ. (Prof. Yuzuru Husimi)

  • Mar. 2004

    M.E. in Functional Materials Science from Saitama Univ.

  • Mar. 2002

    B.E. in Functional Materials Science from Saitama Univ.

Appointments

  • Aug. 2007-Mar. 2010

    Project Researcher, Lab. of Cell Biology, Saitama Univ. (Prof. Takafumi Sakai)

  • Apr. 2010-Mar. 2012

    Project Researcher, Biopolymer Engineering Lab., Saitama Univ. (Prof. Naoto Nemoto)

  • Apr. 2012-Mar. 2015

    Project Researcher, NanoBioDevice Lab., The Univ. of Tokyo (Prof. Takanori Ichiki)

  • Apr. 2015-Present

    Deputy Principal Research Scientist, Innovation Center of NanoMedicine (iCONM)

  • Apr. 2015-Present

    Part-time Lecturer, Saitama Univ.

Performance

Papers

  1. S. Ueno, T. Ichiki
    "Phototriggered control of enzyme reactions toward high-throughput screening on a microwell array chip"
    J. Photopolymer Sci. Technol 30, (2017). accepted
  2. S. Ueno, T. Hirai, S. Sato, M. Biyani, H. Kuramochi, R. Iizuka, T. Akagi, T. Funatsu, T. Ichiki
    "In situ synthesis and immobilization of enzyme molecules on microreactor array chips"
    J. Photopolymer Sci. Technol 28, 719 -726 (2015).
  3. R. Kobayashi, M. Biyani, S. Ueno, S. R. Kumal, H. Kuramochi, T. Ichiki
    "Temperature-controlled microintaglio printing (TC-μIP) for high-resolution micropatterning of transcribed RNA molecules"
    Biosens. Bioelectron. 67, 115-120 (2015).
  4. S. R. Kumal, R. Kobayashi, S. Ueno, T. Ichiki
    "Photo-assisted fabrication of ribosome display microarray"
    J. Photopolymer Sci. Technol. 27, 459-465 (2014).
  5. M. Biyani, Y. Tanaka, S. Sato, S. Ueno, T. Ichiki
    "Evaluation of poly(dimethylsiloxane) microreactors for pattern size miniaturization of microintaglio-printing-based protein microarray"
    Jpn. J. Appl. Phys. 53, 06jl04 (2014).
  6. M. Biyani, J. Moriyasu, Y. Tanaka, S. Sato, S. Ueno, T. Ichiki
    "Microintaglio printing of in situ synthesized proteins enables rapid printing of high-density protein microarrays directly from DNA microarrays"
    Appl. Phys. Express 6, 087001 (2013).
  7. S. R. Kumal, M. Biyani, S. Ueno, T. Akagi, T. Ichiki
    "Simultaneous synthesis and biotinylation of proteins using puromycin-based labeling technology for fabrication of protein array chip"
    Jpn. J. Appl. Phys. 52, 06GK09 (2013).
  8. S. Ueno, S. Kimura, T. Ichiki, N. Nemoto
    "Improvement of a puromycin-linker to extend the selection target varieties in cDNA display method"
    J. Biotechnol. 162, 299-302 (2012).
  9. S. Ueno, A. Ono, R. Kobayashi, Y. Tanaka, S. Sato, M. Biyani, N. Nemoto, T. Ichiki
    "Photoassisted recovery of DNA molecules for on-chip directed evolution"
    J. Photopolymer Sci. Technol. 25, 67-72 (2012).
    THE BEST PAPER AWARD 2013
  10. S. Ueno, S. Yoshida, A. Mondal, K. Nishina, M. Koyama, I. Sakata, K. Miura, Y. Hayashi, N. Nemoto, K. Nishigaki, T. Sakai
    "In vitro selection of a peptide antagonist of growth hormone secretagogue receptor using cDNA display"
    Proc. Natl. Acad. Sci. USA 109, 11121-11126 (2012).
    Highlighted in SciBX (NPG): SciBx 5(27)
  11. S. Ueno, N. Nemoto
    "cDNA display: rapid stabilization of mRNA display"
    Methods Mol. Biol. 805, 113-135 (2012).
  12. N. Nemoto, C. Tsutsui, J. Yamaguchi, S. Ueno, M. Machida, T. Kobayashi, T. Sakai
    "Antagonistic effect of disulfide-rich peptide aptamers selected by cDNA display on interleukin-6-dependent cell proliferation"
    Biochem. Biophys. Res. Commun. 421, 129-133 (2012).
  13. S. Ueno, H. Arai, M. Suzuki, Y. Husimi
    "An mRNA-protein fusion at N-terminus for evolutionary protein engineering"
    Int. J. Biol. Sci. 3, 365-374 (2007).
  14. I. Tabuchi, S. Soramoto, S. Ueno, Y. Husimi
    "Multi-line split DNA synthesis: a novel combinatorial method to make high quality peptide libraries"
    BMC Biotechnol. 4, 19 (2004).

Proceedings

  1. A. Jain, S. Sato, S. Ueno, T. Ichiki
    "High-density self-assembled bead microarray technology for high-throughput aptamer screening"
    Proceedings of The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 900-902 (2015).
  2. S. R. Kumal, R. Kobayashi, S. Ueno, T. Ichiki
    "Ribosome display microarray using μ-intaglio printing and photo-crosslinking without removal of stop codons from DNA"
    Proceedings of The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1817-1819 (2014).
  3. S. Ueno, T. Hirai, A. Tamada, M. Biyani, R. Iizuka, T. Funatsu, T. Ichiki
    "Enzyme-immobilized microwell array for on-chip directed evolution of enzymes"
    Proceedings of The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1802-1804 (2014).
  4. S. Sato, T. Fukuda, T. Hirai, S. Ueno, M. Biyani, T. Akagi, T. Ichiki
    "Fabrication of planar microfluidic device for artificial darwinian selection technology"
    Proceedings of The 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1460-1462 (2013).
  5. S. Ueno, R. Kobayashi, M. Biyani, T. Ichiki
    "Protein-DNA conjugate array chip for on-chip directed evolution"
    Proceedings of The 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1463-1465 (2013).
  6. S. Ueno, A. Ono, R. Kobayashi, Y. Tanaka, S. Sato, M. Biyani, N. Nemoto, T. Ichiki
    "Spot-selective DNA recovery from DNA microarray chips for on-chip directed evolution"
    Proceedings of The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1300-1302 (2012).
  7. S. Soramoto, S. Ueno, I. Tabuchi, Y. Husimi
    "Design of various high quality random libraries for in vitro protein evolution"
    Genome Informatics 13, 527-528 (2002).

Awards

  1. The Society of Photopolymer Science and Technology, The Photopolymer Science and Technology Award, The Best Paper Award 2013,
    "Photoassisted recovery of DNA molecules for on-chip directed evolution"
    S. Ueno, A. Ono, R. Kobayashi, Y. Tanaka, S. Sato, M. Biyani, N. Nemoto, T. Ichiki
    Jun. 2013.

International Patents

  1. Publication No.: WO2015029714
    Title: Production method for protein array or peptide array, identification method for functional protein or functional peptide, protein array or peptide array, and identification kit for functional protein or functional peptide
    Applicants: The University of Tokyo, Nikon Corporation
    Inventors: T. Ichiki, S. Ueno, H. Osawa
  2. Publication No.: WO2014142020
    Title: Nucleic acid linker
    Applicants: The University of Tokyo, Nikon Corporation
    Inventors: T. Ichiki, S. Ueno, M. Biyani, R. Kobayashi, H. Shiono
  3. Publication No.: WO2013065827
    Title: Nucleic acid linker
    Applicants: Saitama University, Nikon Corporation
    Inventors: N. Nemoto, S. Ueno, H. Shiono
  4. Publication No.: WO2013065782
    Title: Protein-immobilizing solid phase, polynucleotide-immobilizing solid phase, and nucleic acid recovery method
    Applicants: The University of Tokyo, Nikon Corporation
    Inventors: S. Ueno, N. Nemoto, T. Ichiki, H. Shiono, H. Osawa

Affiliation Society

The Molecular Biology Society of Japan, The Biophysical Society of Japan