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Ueno, Shingo

Research contents (Research key words)

High functional properties of biomolecules such as enzymes, antibodies, peptides, and nucleic acids are widely used in medical and other various fields. Development or improvement of these biomolecules assist the solution of global issues relating health and medical, food, energy and so on. Functionality of biomolecules have been improved with the evolution of the organisms on the earth. I am developing the technologies that enable to improve or create the functional biomolecules by applying the natural selection theory to biotechnology. Currently, I am focusing on the development of the system for artificial selection of biomolecules based on microarray technologies.

Keywords

Evolutionary Molecular Engineering, In Vitro Selection, Microarray

Biography

Education

• Sep. 2007

  Ph.D. in Evolutionary Molecular Engineering from Saitama Univ. (Prof. Yuzuru Husimi)

• Mar. 2004

  M.E. in Functional Materials Science from Saitama Univ.

• Mar. 2002

  B.E. in Functional Materials Science from Saitama Univ.

Appointments

• Aug. 2007-Mar. 2010

  Project Researcher, Lab. of Cell Biology, Saitama Univ. (Prof. Takafumi Sakai)

• Apr. 2010-Mar. 2012

  Project Researcher, Biopolymer Engineering Lab., Saitama Univ. (Prof. Naoto Nemoto)

• Apr. 2012-Mar. 2015

  Project Researcher, NanoBioDevice Lab., The Univ. of Tokyo (Prof. Takanori Ichiki)

• Apr. 2015-Mar. 2019

  Part-time Lecturer, Saitama Univ.

• Apr. 2015-Present

  Deputy Principal Research Scientist, Innovation Center of NanoMedicine (iCONM)

Performance

Papers

1. B. S. Kim, M. Naito, R. Kamegawa, H. J. Kim, R. Iizuka, T. Funatsu, S. Ueno, T. Ichiki, A. Kishimura, K. Miyata, S. Sato, S. Ueno, T. Ichiki, A. Kishimura, and K. Miyata,
   "Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular uptake and gene knockdown"
   Chem. Commun. 56, 9477-9480 (2020).
2. S. Sato, S. Ueno, T. Ichiki
   "A surface modification process for silica microreactor array chips to seal aqueous solutions "
   Jpn. J. Appl. Phys 59, SIIK01 (2020).
3. S. Ueno, M. Shioya, T. Ichiki
   "Fluorometric measurement of adenosine 5′-triphosphate using exonuclease V activity"
   J. Photopolymer Sci. Technol 31, 699-704 (2018).
4. S. Ueno, T. Ichiki
   "Phototriggered control of enzyme reactions toward high-throughput screening on a microwell array chip"
   J. Photopolymer Sci. Technol 30, 657-660 (2017).
5. S. Ueno, T. Hirai, S. Sato, M. Biyani, H. Kuramochi, R. Iizuka, T. Akagi, T. Funatsu, T. Ichiki
   "In situ synthesis and immobilization of enzyme molecules on microreactor array chips"
   J. Photopolymer Sci. Technol 28, 719 -726 (2015).
6. R. Kobayashi, M. Biyani, S. Ueno, S. R. Kumal, H. Kuramochi, T. Ichiki
   "Temperature-controlled microintaglio printing (TC-µIP) for high-resolution micropatterning of transcribed RNA molecules"
   Biosens. Bioelectron. 67, 115-120 (2015).
7. S. R. Kumal, R. Kobayashi, S. Ueno, T. Ichiki
   "Photo-assisted fabrication of ribosome display microarray"
   J. Photopolymer Sci. Technol. 27, 459-465 (2014).
8. M. Biyani, Y. Tanaka, S. Sato, S. Ueno, T. Ichiki
   "Evaluation of poly(dimethylsiloxane) microreactors for pattern size miniaturization of microintaglio-printing-based protein microarray"
   Jpn. J. Appl. Phys. 53, 06jl04 (2014).
9. M. Biyani, J. Moriyasu, Y. Tanaka, S. Sato, S. Ueno, T. Ichiki
   "Microintaglio printing of in situ synthesized proteins enables rapid printing of high-density protein microarrays directly from DNA microarrays"
   Appl. Phys. Express 6, 087001 (2013).
10. S. R. Kumal, M. Biyani, S. Ueno, T. Akagi, T. Ichiki
    "Simultaneous synthesis and biotinylation of proteins using puromycin-based labeling technology for fabrication of protein array chip"
    Jpn. J. Appl. Phys. 52, 06GK09 (2013).
11. S. Ueno, S. Kimura, T. Ichiki, N. Nemoto
    "Improvement of a puromycin-linker to extend the selection target varieties in cDNA display method"
    J. Biotechnol. 162, 299-302 (2012).
12. S. Ueno, A. Ono, R. Kobayashi, Y. Tanaka, S. Sato, M. Biyani, N. Nemoto, T. Ichiki
    "Photoassisted recovery of DNA molecules for on-chip directed evolution"
    J. Photopolymer Sci. Technol. 25, 67-72 (2012).
    THE BEST PAPER AWARD 2013
13. S. Ueno, S. Yoshida, A. Mondal, K. Nishina, M. Koyama, I. Sakata, K. Miura, Y. Hayashi, N. Nemoto, K. Nishigaki, T. Sakai
    "In vitro selection of a peptide antagonist of growth hormone secretagogue receptor using cDNA display"
    Proc. Natl. Acad. Sci. USA 109, 11121-11126 (2012).
    Highlighted in SciBX (NPG): SciBx 5(27)
14. S. Ueno, N. Nemoto
    "cDNA display: rapid stabilization of mRNA display"
    Methods Mol. Biol. 805, 113-135 (2012).
15. N. Nemoto, C. Tsutsui, J. Yamaguchi, S. Ueno, M. Machida, T. Kobayashi, T. Sakai
    "Antagonistic effect of disulfide-rich peptide aptamers selected by cDNA display on interleukin-6-dependent cell proliferation"
    Biochem. Biophys. Res. Commun. 421, 129-133 (2012).
16. S. Ueno, H. Arai, M. Suzuki, Y. Husimi
    "An mRNA-protein fusion at N-terminus for evolutionary protein engineering"
    Int. J. Biol. Sci. 3, 365-374 (2007).
17. I. Tabuchi, S. Soramoto, S. Ueno, Y. Husimi
    "Multi-line split DNA synthesis: a novel combinatorial method to make high quality peptide libraries"
    BMC Biotechnol. 4, 19 (2004).

Proceedings

1. S. Ueno, Y. Shirakata, M. Shioya, S. Sato, S. Tsuchiya, T. Ichiki
   "Transpeptidase-mediated in-situ covalent immobilization of cell-free synthesized enzyme for on-chip directed evolution"
   Proceedings of the 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, 870-871 (2019).
2. S. Ueno, T. Ichiki
   "Spatiotemporal phototriggered control of biochemical reactions for on-chip ultrahigh-throughput screening of enzyme activities"
   Proceedings of the 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1301-1302 (2017).
3. R. Wakai, S. Ueno, T. Ichiki
   "In situ covalent immobilization of protein on a chip by sortase-mediated peptide ligation"
   Proceedings of the 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1299-1300 (2017).
4. A. Jain, S. Sato, S. Ueno, T. Ichiki
   "High-density self-assembled bead microarray technology for high-throughput aptamer screening"
   Proceedings of the 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 900-902 (2015).
5. S. R. Kumal, R. Kobayashi, S. Ueno, T. Ichiki
   "Ribosome display microarray using µ-intaglio printing and photo-crosslinking without removal of stop codons from DNA"
   Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1817-1819 (2014).
6. S. Ueno, T. Hirai, A. Tamada, M. Biyani, R. Iizuka, T. Funatsu, T. Ichiki
   "Enzyme-immobilized microwell array for on-chip directed evolution of enzymes"
   Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1802-1804 (2014).
7. S. Sato, T. Fukuda, T. Hirai, S. Ueno, M. Biyani, T. Akagi, T. Ichiki
   "Fabrication of planar microfluidic device for artificial darwinian selection technology"
   Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1460-1462 (2013).
8. S. Ueno, R. Kobayashi, M. Biyani, T. Ichiki
   "Protein-DNA conjugate array chip for on-chip directed evolution"
   Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1463-1465 (2013).
9. S. Ueno, A. Ono, R. Kobayashi, Y. Tanaka, S. Sato, M. Biyani, N. Nemoto, T. Ichiki
   "Spot-selective DNA recovery from DNA microarray chips for on-chip directed evolution"
   Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1300-1302 (2012).
10. S. Soramoto, S. Ueno, I. Tabuchi, Y. Husimi
    "Design of various high quality random libraries for in vitro protein evolution"
    Genome Informatics 13, 527-528 (2002).

Awards

1. The Society of Photopolymer Science and Technology, The Photopolymer Science and Technology Award, The Best Paper Award 2013,
   "Photoassisted recovery of DNA molecules for on-chip directed evolution"
   S. Ueno, A. Ono, R. Kobayashi, Y. Tanaka, S. Sato, M. Biyani, N. Nemoto, T. Ichiki
   Jun. 2013.

International Patents

1. Publication No.: WO2020095985
   Title: Peptide-nucleic acid complex
   Applicants: Kawasaki Institute of Industrial Promotion
   Inventors: S. Ueno,T. Ichiki
2. Publication No.: WO2020013318
   Title: Substrate having modification layer and method for manufacturing same
   Applicants: Kawasaki Institute of Industrial Promotion
   Inventors: S. Sato, S. Ueno,T. Ichiki
3. Publication No.: WO2015029714
   Title: Production method for protein array or peptide array, identification method for functional protein or functional peptide, protein array or peptide array, and identification kit for functional protein or functional peptide
   Applicants: The University of Tokyo, Nikon Corporation
   Inventors: T. Ichiki, S. Ueno, H. Osawa
4. Publication No.: WO2014142020
   Title: Nucleic acid linker
   Applicants: The University of Tokyo, Nikon Corporation
   Inventors: T. Ichiki, S. Ueno, M. Biyani, R. Kobayashi, H. Shiono
5. Publication No.: WO2013065827
   Title: Nucleic acid linker
   Applicants: Saitama University, Nikon Corporation
   Inventors: N. Nemoto, S. Ueno, H. Shiono
6. Publication No.: WO2013065782
   Title: Protein-immobilizing solid phase, polynucleotide-immobilizing solid phase, and nucleic acid recovery method
   Applicants: The University of Tokyo, Nikon Corporation
   Inventors: S. Ueno, N. Nemoto, T. Ichiki, H. Shiono, H. Osawa

Affiliation Society

The Molecular Biology Society of Japan, The Biophysical Society of Japan