Research Activities


Itaka Lab (2015-present)

Original Paper

  1. Uchida S, Yanagihara K, Matsui A, Kataoka K, Itaka K. mRNA as a Tool for Gene Transfection in 3D Cell Culture for Future Regenerative Therapy. Micromachines 11(4): 426, 2020
  2. Crowley ST, Fukushima Y, Uchida S, Kataoka K, Itaka K. Enhancement of Motor Function Recovery after Spinal Cord Injury in Mice by Delivery of Brain-Derived Neurotrophic Factor mRNA. Mol Ther Nucleic Acids. 17:465-476, 2019
  3. Lin CY, Crowley S, Uchida S, Komaki Y, Kataoka K, Itaka K. Treatment of intervertebral disk disease by administration of messenger RNA encoding a cartilage-anabolic transcription factor. Mol Ther Nucleic Acids 16: 162-171, 2019
  4. Crowley S, Kataoka K, Itaka K. Combined CatWalk Index: an improved method to measure mouse motor function using the automated gait analysis system. BMC Research Notes11:263, 2018
  5. Yanagihara K, Uchida S, Ohba S, Kataoka K, Itaka K. Treatment of Bone Defects by Transplantation of Genetically Modified Mesenchymal Stem Cell Spheroids. Molecular Therapy - Methods & Clinical Development. 9: 358-366, 2018
  6. Matsui A, Uchida S, Hayashi A, Kataoka K, Itaka K. Prolonged engraftment of transplanted hepatocytes in the liver by transient pro-survival factor supplementation using ex vivo mRNA transfection. J Control Release. 285; 1-11, 2018
  7. Uchida S, Yoshinaga N, Yanagihara K, Yuba E, Kataoka K, Itaka K. Designing immunostimulatory double stranded messenger RNA with maintained translational activity through hybridization with poly A sequences for e?ective vaccination. Biomaterials. 150:162-170, 2018
  8. Anraku Y, Kuwahara H, Fukusato Y, Mizoguchi A, Ishii T, Nitta K, Matsumoto Y, Toh K, Miyata K, Uchida S, Nishina K, Osada K, Itaka K, Nishiyama N, Mizusawa H, Yamasoba T, Yokota T, Kataoka K. Glycaemic control boosts glucosylated nanocarrier crossing the BBB into the brain. Nature Communications 8: 1001, 2017
  9. Perche F, Uchida S, Akiba H, Lin CY, Ikegami M, Dirisala A, Nakashima T, Itaka K, Tsumoto K, Kataoka K. Improved Brain Expression of Anti-Amyloid β scFv by Complexation of mRNA Including a Secretion Sequence with PEG-based Block Catiomer. Curr Alzheimer Res. 14(3):295-302. 2017
  10. Lin CY, Perche F, Ikegami M, Uchida S, Kataoka K, Itaka K. Messenger RNA-based therapeutics for brain diseases: An animal study for augmenting clearance of beta-amyloid by intracerebral administration of neprilysin mRNA loaded in polyplex nanomicelles. J Control Release. 235:268-75, 2016
  11. Uchida S, Hayakawa K, Ogata T, Tanaka S, Kataoka K, Itaka K. Treatment of spinal cord injury by an advanced cell transplantation technology using brain-derived neurotrophic factor-transfected mesenchymal stem cell spheroids. Biomaterials. 109:1-11, 2016
  12. Uchida H, Itaka K, Uchida S, Ishii T, Suma T, Miyata K, Oba M, Nishiyama N, Kataoka K. Synthetic Polyamines to Regulate mRNA Translation through the Preservative Binding of Eukaryotic Initiation Factor 4E to the Cap Structure. J Am Chem Soc. 138(5):1478-81, 2016
  13. Uchida S, Kinoh H, Ishii T, Matsui A, Tockary TA, Takeda KM, Uchida H, Osada K, Itaka K, Kataoka K. Systemic delivery of messenger RNA for the treatment of pancreatic cancer using polyplex nanomicelles with a cholesterol moiety. Biomaterials. 82:221-8, 2016
  14. Aini H, Itaka K, Fujisawa A, Uchida H, Uchida S, Fukushima S, Kataoka K, Saito T, Chung U, Ohba S. Messenger RNA delivery of a cartilage-anabolic transcription factor as a disease-modifying strategy for osteoarthritis treatment. Sci Rep. 6: 18743, 2016
  15. Okuda T, Suzuki Y, Kobayashi Y, Ishii T, Uchida S, Itaka K, Kataoka K, Okamoto H. Development of Biodegradable Polycation-Based Inhalable Dry Gene Powders by Spray Freeze Drying. Pharmaceutics. 7(3): 233-54, 2015
  16. Matsui A, Uchida S, Ishii T, Itaka K, Kataoka K. Messenger RNA-based therapeutics for the treatment of apoptosis-associated diseases. Sci Rep. 5:15810, 2015
  17. Li Y, Osada K, Chen Q, Tockary TA, Dirisala A, Takeda KM, Uchida S, Nagata K, Itaka K, Kataoka K. Toroidal Packaging of pDNA into Block Ionomer Micelles Exerting Promoted in Vivo Gene Expression. Biomacromolecules. 16(9): 2664-71, 2015
  18. Itaka K, Uchida S, Matsui A, Yanagihara K, Ikegami M, Endo T, Ishii T, Kataoka K. Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation. J Vis Exp . 101: e52384, 2015
  19. Uchida S, Kataoka K, Itaka K. Screening of mRNA Chemical Modification to Maximize Protein Expression with Reduced Immunogenicity. Pharmaceutics. 7(3):137-51, 2015
  20. Chen Q, Osada K, Pennisi M, Uchida S, Tockary TA, Dirisala A, Li Y, Takeda KM, Oniyanagi S, Itaka K, Kataoka K. A tadpole-shaped gene carrier with distinct phase segregation in a ternary polymeric micelle. Soft Matter. 11(14):2718-22, 2015
  21. Baba M, Itaka K, Kondo K, Yamasoba T, Kataoka K. Treatment of neurological disorders by introducing mRNA in vivo using polyplex nanomicelles. J Control Release. 201:41-8,2015
  22. Hayakawa K, Uchida S, Ogata T, Tanaka S, Kataoka K, Itaka K. Intrathecal injection of a therapeutic gene-containing polyplex to treat spinal cord injury. J Control Release. 197:1-9,2015

Review [Japanese]

  1. 内田智士、位髙啓史.相補鎖RNAオリゴマーを用いたmRNA修飾と,そのmRNA送達,ワクチンへの応用.バイオマテリアル-生体材料-. 38-2: 106-111, 2020
  2. 位髙啓史.医療のニーズに応えるバイオマテリアル.バイオマテリアル-生体材料-. 38-2: 89, 2020
  3. 位髙啓史.mRNA医薬に利用されるキャリア開発:ナノミセル型キャリア.Drug Delivery System 35-1, 27-34, 2020
  4. 荒戸 照世, 位髙 啓史,秋永 士朗,佐藤 秀昭,山口 照英,真木 一茂,内田 恵理子,吉田 徳幸,井上 貴雄.mRNA医薬品の品質・安全性評価の考え方.医薬品医療機器レギュラトリーサイエンス, PMDRS, 50(6), 300-306, 2019
  5. 位髙啓史、秋永士朗、井上貴雄.mRNA医薬開発の世界的動向.医薬品医療機器レギュラトリーサイエンス, PMDRS, 50(5), 242-249, 2019
  6. 位髙啓史 『mRNA医薬の研究開発動向と将来展望』 医薬ジャーナル55(2): 627-632, 2019
  7. 位高啓史、福島雄大 『【脳神経系へ挑むバイオマテリアル】mRNA 医薬を用いた脳神経疾患の治療戦略』 バイオマテリアル-生体材料- 36(3): 220-223, 2018
  8. 位髙啓史 『メッセンジャーRNA医薬を実現するDDS開発と疾患治療への応用』 Drug Delivery System(DDS)31(4):343-351, 2016
  9. 位髙啓史 『メッセンジャーRNA医薬の開発戦略』 日薬理誌(Folia Pharmacol.Jpn)148:190-196, 2016.
  10. Itaka K. 『Introduction to Special Issue: A New Paradigm of Gene Therapy』 Pharmaceutics 8(1), 1; 2016
  11. 位髙啓史 『片岡一則 ナノDDSの再生医療への展開:mRNAデリバリーによる生体内細胞制御』 細胞工学 34(10): 946-951,2015
  12. 位髙啓史 『整形外科におけるDDS:再生医療への応用』 BIO Clinica 30(7) (特集:DDS技術の医療応用への新展開), 35-40, 2015.
  13. 位髙啓史、片岡一則 『超分子ナノマシンによる薬物・遺伝子のピンポイントデリバリー』 泌尿器外科28(臨増),557-558,2015
  14. 位髙啓史 『整形トピックス:脊髄組織で蛋白発現持続を可能とするmRNAデリバリーシステムの開発と脊髄損傷治療への展開』 臨床雑誌整形外科65(12): 1248, 20141.11.1(11月号)

Miscellaneous [English]

  1. 位髙啓史.「認知症の早期診断技術と進行抑制/予防薬・機能性食品の開発」(株)技術情報協会 2019年4月26日発刊
  2. 位髙啓史.「三次元培養における培養手法と周辺技術動向」(株)情報機構 2019年4月17日発行