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Members

Iizuka, Ryo

Research contents (Research key words)

Research contents

1. Development of liquid biopsy platforms for early cancer detection
2. Exploration and creation of novel functional biomolecules using a microdroplet platform
3. Function and structure analysis of proteins and supramolecular complexes using single-molecule imaging and force spectroscopy

Keywords

Microarray, Directed evolution, Microdroplet, Single-molecule imaging, Optical tweezers.

Biography

Education

• 2001.3

  B.E., Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology

• 2002.3

  M.E., Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology

• 2004.9

  Ph.D., Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology

Employment

• 2004.10–2005.4

  Government-industry-academia collaboration researcher, Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology

• 2005.5–2006.3

  Specially Appointed Research Associate, Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology

• 2006.4–2009.2

  Research Fellowship for Young Scientists (PD), Japan Society for the Promotion of Science

• 2009.3–2012.10

  Specially Appointed Assistant Professor, Graduate School of Pharmaceutical Sciences, The University of Tokyo

• 2012.11–2020.3

  Assistant Professor, Graduate School of Pharmaceutical Sciences, The University of Tokyo

• 2020.4–present

  Assistant Professor, Department of Biological Sciences, Graduate School of Science, The University of Tokyo

• 2016.2–present

  Visiting Researcher, Innovation Center of NanoMedicine

Performance

Recent papers

1. Kim, B. S., Naito, M., Kamegawa, R., Kim, H. J., Iizuka, R., Funatsu, T., Ueno, S., Ichiki, T., Kishimura, A., and Miyata, K. (2020). Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular uptake and gene knockdown. Chem. Commun. 56, 9477–9480 (2020)
2. Muta, M., Iizuka, R., Niwa, T., Guo, Y., Taguchi, H., and Funatsu, T.: Nascent SecM chain interacts with outer ribosomal surface to stabilize translation arrest. Biochem. J. 477, 557–566 (2020)
3. Masubuchi, T., Endo, M., Iizuka, R., Iguchi, A., Yoon, D. H., Sekiguchi, T., Qi, H., Iinuma, R., Miyazono, Y., Shoji, S., Funatsu, T., Sugiyama, H., Harada, Y., Ueda, T., and Tadakuma, H. “Construction of integrated gene logic-chip.” Nat. Nanotechnol. 13, 933–940 (2018)
4. Iizuka, R., and Funatsu, T. “Chaperonin GroEL uses asymmetric and symmetric reaction cycles in response to the concentration of non-native substrate proteins.” Biophys. Physicobiol. 13, 63–69 (2016)
5. Nakamura, K., Iizuka, R., Nishi, S., Yoshida, T., Hatada, Y., Takaki, Y., Iguchi, A., Yoon, D. H., Sekiguchi, T., Shoji, S., and Funatsu, T. “Culture-independent method for identification of microbial enzyme-encoding genes by activity-based single-cell sequencing using a water-in-oil microdroplet platform.” Sci. Rep. 6, 22259 (2016)
6. Iizuka, R., Ueno, T., and Funatsu, T. “Detection and quantification of microRNAs by ligase-assisted sandwich hybridization on a microarray.” Methods Mol. Biol. 1368, 53–65 (2016)
7. Ueno, S., Hirai, T., Sato, S., Biyani, M., Kuramochi, H., Iizuka, R., Akagi, T., Funatsu, T., and Ichiki, T. “In situ synthesis and immobilization of enzyme molecules on microreactor array chips.” J. Photopolymer Sci. Technol. 28, 719–726 (2015)
8. Yang, Z., Iizuka, R., and Funatsu, T. “Nascent SecM chain outside the ribosome reinforces translation arrest.” PLoS ONE 10, e0122017 (2015)
9. Ono, T., Iizuka, R., Akagi, T., Funatsu, T., and Ichiki, T. “Optical observation of deep bulk damage in amorphous perfluorocarbon films produced by UV photons emitted from low-pressure argon plasmas.” J. Photopolymer Sci. Technol. 27, 393–398 (2014)
10. Takei, Y., Iizuka, R., Ueno, T., and Funatsu, T. “Single-molecule observation of protein folding in symmetric GroEL-(GroES)2 complexes.” J. Biol. Chem. 287, 41118–41125 (2012)
11. Masuda, T., Petrov, A., Iizuka, R., Funatsu, T., Puglisi, J. D., and Uemura, S. “Initiation factor 2, tRNA, and 50S subunits cooperatively stabilize mRNAs on the ribosome during initiation.” Proc. Natl. Acad. Sci. U. S. A. 109, 4881–4885 (2012)
12. Ono, T., Iizuka, R., Akagi, T., Funatsu, T., and Ichiki, T. “Damage-free fabrication of perfluoropolymer microaperture array device for single-molecule imaging.” Trans. Mater. Res. Soc. Jpn. 36, 553–556 (2011)
13. Haneoka, M., Shirasaki, Y., Sugino, H., Aoki, T., Arakawa, T., Ozaki, K., Yoon D. H., Ishii, N., Iizuka, R., Shoji, S., and Funatsu, T. “Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition.” Sensor Actuat. B-Chem. 159, 314–320 (2011)
14. Iizuka, R., Funatsu, T., and Uemura, S. “Real-time single-molecule observation of green fluorescent protein synthesis by immobilized ribosomes.” Methods Mol. Biol. 778, 215–228 (2011)
15. Iizuka, R., Ueno, T., Morone, N., and Funatsu, T. “Single-molecule fluorescence polarization study of conformational change in archaeal group II chaperonin.” PLoS ONE 6, e22253 (2011)
16. Iizuka, R., Yamagishi-Shirasaki, M., and Funatsu, T. “Kinetic study of de novo chromophore maturation of fluorescent proteins.” Anal. Biochem. 414, 173–178 (2011)

Affiliation Society

The Japanese Biochemical Society, Protein Science Society of Japan, The Biophysical Society of Japan, Japanese Society for Extremophiles, The Pharmaceutical Society of Japan, Japanese Society of Enzyme Engineering, Japan Society for Environmental Biotechnology.